Establishment of an Academic Tissue Microarray Platform as a Tool for Soft Tissue Sarcoma Research

Soft tissue sarcoma (STS) is a heterogeneous family of rare mesenchymal tumors, characterized by histopathological and molecular diversity. Tissue microarray (TMA) tool that allows performing research in orphan diseases more efficient cost-effective way. TMAs are paraffin blocks consisting multiple small representative cores from biological samples, for example, donors, diverse sites disease, or different diseases. In 2015, we began constructing using archival tumor material STS patients. Specimens were well annotated terms diagnosis, treatment, clinical follow-up the donors. Each TMA block contains duplicate triplicate 1.0–1.5 mm areas selected pathologists. The construction was performed with Grand Master (3DHistech). So far, have established disease-specific 7 subtypes: gastrointestinal stromal (72 cases included array), alveolar soft part (n = 12 + 47), clear cell 22 32), leiomyosarcoma 55), liposarcoma 42), inflammatory myofibroblastic 21), rhabdomyosarcoma 24). We also constructed multisarcoma covering number important subtypes on arrays screening purposes, namely, angiosarcoma, dedifferentiated liposarcoma, pleomorphic myxoid leiomyosarcoma, malignant peripheral nerve sheath tumor, myxofibrosarcoma, rhabdomyosarcoma, synovial sarcoma, undifferentiated 7–11 individual per subtype. currently expanding list additional entities, considering heterogeneity this tumors. Our extensive platform suitable rapid morphological, immunohistochemical, characterization as identification potential novel diagnostic markers drug targets. It readily available collaborative projects partners.